5YFI

Crystal structure of the anti-human prostaglandin E receptor EP4 antibody Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.703, 60.784, 200.182
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.935 - 1.848
R-factor	0.1957
Rwork	0.194
R-free	0.23150
RMSD bond length	0.005
RMSD bond angle	0.766
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.848
Number of reflections	81114
<I/σ(I)>	9
Completeness [%]	98.8
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	15% PEG 4000, 200 mM zinc acetate, 100 mM MES (pH 6.5), 100 mM guanidine hydrochloride