5YF4
A kinase complex MST4-MOB4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.469, 33.997, 61.023 |
Unit cell angles | 90.00, 122.67, 90.00 |
Refinement procedure
Resolution | 30.038 - 1.897 |
R-factor | 0.1572 |
Rwork | 0.153 |
R-free | 0.19770 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.246 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.965 |
High resolution limit [Å] | 1.897 | 1.897 |
Rmerge | 0.109 | 0.798 |
Rmeas | 0.119 | 0.881 |
Rpim | 0.046 | 0.365 |
Number of reflections | 12086 | 1123 |
<I/σ(I)> | 15.5 | |
Completeness [%] | 98.9 | 99 |
Redundancy | 6.4 | 5.5 |
CC(1/2) | 0.715 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M HEPES, pH 7.5, 30% PEG 1000 |