5YEL
Crystal structure of CTCF ZFs6-11-gb7CSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.993, 69.631, 84.445 |
| Unit cell angles | 90.00, 116.25, 90.00 |
Refinement procedure
| Resolution | 37.874 - 2.960 |
| R-factor | 0.2687 |
| Rwork | 0.268 |
| R-free | 0.28560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.870 | 3.010 |
| High resolution limit [Å] | 2.900 | 2.960 |
| Rmerge | 0.087 | 0.653 |
| Rpim | 0.029 | 0.221 |
| Number of reflections | 30548 | |
| <I/σ(I)> | 30.6 | 4.3 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 9.6 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 0.1M Bis-tris pH 5.6-6.0, 0.1M Sodium chloride, 17-20% PEG3350 |
