5YEK
Crystal structure of BioQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97776 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 37.905, 193.941, 54.000 |
| Unit cell angles | 90.00, 100.20, 90.00 |
Refinement procedure
| Resolution | 48.485 - 2.191 |
| R-factor | 0.2137 |
| Rwork | 0.212 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yej |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.238 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.190 |
| Number of reflections | 19546 |
| <I/σ(I)> | 9.5 |
| Completeness [%] | 99.3 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 3350, Bis-Tris, ammonium sulfate |
