5YEF
Crystal structure of CTCF ZFs2-8-Hs5-1aE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.115, 143.064, 90.233 |
| Unit cell angles | 90.00, 119.69, 90.00 |
Refinement procedure
| Resolution | 45.057 - 2.807 |
| R-factor | 0.2253 |
| Rwork | 0.224 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.333 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.140 | 2.860 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.095 | 0.628 |
| Rpim | 0.045 | 0.359 |
| Number of reflections | 45830 | |
| <I/σ(I)> | 19.3 | 3.44 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 0.1 M Bis-tris pH 5.6-6.0, 0.2 M Sodium chloride and 17-24% PEG 3350 |
