5YDU
Crystal structure of Utp30
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97776 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 72.364, 90.880, 157.232 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.939 - 2.646 |
R-factor | 0.2036 |
Rwork | 0.200 |
R-free | 0.26550 |
Structure solution method | SIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.081 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.646 | 2.646 |
Rmerge | 0.168 | 0.765 |
Number of reflections | 15291 | 1432 |
<I/σ(I)> | 21.4 | 2 |
Completeness [%] | 98.7 | 95.4 |
Redundancy | 11.4 | 8.4 |
CC(1/2) | 0.768 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.45 M tri-sodium citrate dehydrate and 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.5 |