5YDG

Crystal structure of the Arabidopsis thaliana chloroplast RNA editing factors 2(MORF2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	38.209, 85.880, 86.559
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.644 - 2.405
R-factor	0.2059
Rwork	0.204
R-free	0.24730
RMSD bond length	0.005
RMSD bond angle	0.903
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (MR)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.106	0.053	0.445
Rmeas	0.124	0.063	0.522
Rpim	0.063	0.033	0.266
Number of reflections	11069	1207	1118
<I/σ(I)>	7.4
Completeness [%]	95.4	94.1	99.7
Redundancy	3.7	3.4	3.8
CC(1/2)		0.993	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.1 M Bis-Tris pH 6.5, 25% (w/v) PEG 3350