5YDF

Crystal structure of a disease-related gene, hCDC73(1-100)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 1 21 1
Unit cell lengths	28.590, 53.504, 65.734
Unit cell angles	90.00, 95.66, 90.00

Refinement procedure

Resolution	19.489 - 1.399
R-factor	0.1372
Rwork	0.135
R-free	0.17420
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.361
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.500
High resolution limit [Å]	1.400
Number of reflections	38451
<I/σ(I)>	41.92
Completeness [%]	98.9
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Tris, magnesium chloride
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Tris, magnesium chloride