5YD5
Crystal structure of the scFv antibody 4B08 with epitope peptide (mutation N3A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 61.620, 81.900, 114.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.300 - 1.960 |
| R-factor | 0.20548 |
| Rwork | 0.203 |
| R-free | 0.25789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yd3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.548 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.129 | 0.425 |
| Rpim | 0.065 | 0.174 |
| Number of reflections | 40361 | 2555 |
| <I/σ(I)> | 10 | 3.7 |
| Completeness [%] | 95.6 | 87.1 |
| Redundancy | 7.7 | 6.2 |
| CC(1/2) | 0.997 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 100 mM BIS-TRIS 1.8 M ammonium sulphate |
