5YC3

Crystal structure of AL3 PHD finger bound to H3K4me2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 41 2 2
Unit cell lengths	30.921, 30.921, 157.029
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.257 - 2.601
R-factor	0.1983
Rwork	0.191
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fsa
RMSD bond length	0.003
RMSD bond angle	0.670
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.189	0.055	0.902
Rmeas	0.197	0.058	0.935
Rpim	0.053	0.017	0.244
Number of reflections	2763	191	122
<I/σ(I)>	4.8
Completeness [%]	100.0	100	100
Redundancy	13.4	10.1	14.4
CC(1/2)		0.999	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	1.8 M Ammonium citrate tribasic, pH 7.0.