5YC2
Crystal structure of inner membrane protein Bqt4 in complex with telomeric protein Rap1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.154, 51.293, 79.669 |
| Unit cell angles | 94.92, 100.56, 105.00 |
Refinement procedure
| Resolution | 39.056 - 2.704 |
| R-factor | 0.2044 |
| Rwork | 0.202 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ybx |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.481 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.056 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.324 | |
| Rmeas | 0.391 | |
| Rpim | 0.216 | |
| Number of reflections | 12761 | |
| <I/σ(I)> | 1.7 | 2.428 |
| Completeness [%] | 98.0 | 94.8 |
| Redundancy | 1 | 2.9 |
| CC(1/2) | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES sodium pH 7.5, 10% v/v 2-Propanol, 20% w/v Polyethyleneglycol 4000. |
