5YBU

Structure of the KANK1 ankyrin domain in complex with KIF21A peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	37.648, 51.624, 137.609
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.335 - 1.890
R-factor	0.2249
Rwork	0.222
R-free	0.25290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hbd
RMSD bond length	0.010
RMSD bond angle	1.227
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2722)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.335
High resolution limit [Å]	1.890
Number of reflections	22149
<I/σ(I)>	29.3
Completeness [%]	99.5
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.05M magnesium formate, 21% PEG 3350