5YBM

Fe(II)/(alpha)ketoglutarate-dependent dioxygenase PrhA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2016-11-03
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 62
Unit cell lengths	172.834, 172.834, 44.705
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.208 - 2.115
R-factor	0.1894
Rwork	0.188
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ybl
RMSD bond length	0.007
RMSD bond angle	0.828
Data scaling software	Aimless
Phasing software	PHENIX ((1.10.1_2155))
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	44042
<I/σ(I)>	9.1
Completeness [%]	99.8
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, lithium citrate