5YBJ
Structure of apo KANK1 ankyrin domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-01-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.903, 47.602, 137.745 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.990 - 2.341 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.24170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.229 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2722) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.990 |
High resolution limit [Å] | 2.340 |
Number of reflections | 12363 |
<I/σ(I)> | 28.1 |
Completeness [%] | 99.7 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1M HEPES, pH 7.5, 0.2M Lithium sulfate monohydrate, 25% PEG 3350 |