5YB4
Crystal structure of HP23LN36KR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 51.280, 51.280, 142.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.150 - 2.500 |
R-factor | 0.1916 |
Rwork | 0.188 |
R-free | 0.26660 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.902 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 35.150 |
High resolution limit [Å] | 2.500 |
Number of reflections | 7140 |
<I/σ(I)> | 15.8 |
Completeness [%] | 100.0 |
Redundancy | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | (A12) 10%(w/v) PEG 8000, 1M HEPES, PH=7.5, 8%(v/v) Ethylene glycol 13 (B1) 8%(w/v) PEG 4000, 1M Sodium acetate PH=4.6 |