5YAE
Ferulic acid esterase from Streptomyces cinnamoneus at 2.4 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-05 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 2.7 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.270, 71.290, 87.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.320 - 2.400 |
R-factor | 0.18248 |
Rwork | 0.180 |
R-free | 0.22026 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.355 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.320 | 2.470 |
High resolution limit [Å] | 2.340 | 2.400 |
Rmerge | 0.070 | 0.190 |
Number of reflections | 14597 | |
<I/σ(I)> | 42.9 | 10.6 |
Completeness [%] | 93.9 | 87.9 |
Redundancy | 30.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | ammonium sulfate, sodium cacodylate trihydrate (pH 6.5), PEG 8000, glycerol |