5YAC
Crystal structure of WT Trm5b from Pyrococcus abyssi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | Agilent SuperNova |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-13 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.5406 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.444, 88.567, 95.164 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.150 - 3.300 |
R-factor | 0.24386 |
Rwork | 0.242 |
R-free | 0.28847 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hjm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.269 |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.150 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.204 | 0.980 |
Number of reflections | 5639 | 812 |
<I/σ(I)> | 6.3 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 4.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 12% (w/v) PEG 3350, 100 mM HEPES (pH 7.5), 100 mM Ca(OAc)2 and 100 mM KCl |