5YA6

Crystal structure of archaeal flagellin FlaB1 from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	37.090, 73.660, 107.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 1.500
R-factor	0.17408
Rwork	0.173
R-free	0.20000
Structure solution method	MIR
RMSD bond length	0.019
RMSD bond angle	1.815
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	1.580
High resolution limit [Å]	1.500	1.500
Rmerge		0.325
Number of reflections	46269
<I/σ(I)>	12.2	3.3
Completeness [%]	96.8	92.9
Redundancy	4.3	4.3
CC(1/2)		0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283	32.5% PEG4000