5YA0

Crystal structure of LsrK and HPr complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-17
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	101.156, 101.156, 344.323
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.004 - 2.997
R-factor	0.2007
Rwork	0.199
R-free	0.23870
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.973
Data scaling software	HKL-2000 (1.9_1692)
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.072
High resolution limit [Å]	2.700	2.997
Number of reflections	41480
<I/σ(I)>	22.2
Completeness [%]	98.3
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	5~20% (v/v) PEG-400, 0.1 M sodium phosphate-citrate (pH 4.2), and 0.2 M lithium sulfate