5Y9Q
Crystal structure of the CcpE regulatory domain at 1.95 Angstrom from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9826 |
| Spacegroup name | H 3 |
| Unit cell lengths | 111.667, 111.667, 123.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.207 - 1.953 |
| R-factor | 0.1791 |
| Rwork | 0.177 |
| R-free | 0.22040 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.378 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.104 | 0.104 |
| Number of reflections | 75836 | 41088 |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 8.3 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 287 | 4 M Sodium formate |
