5Y9C
Crystal structure of HPV58 pentamer in complex with the Fab fragment of antibody A12A3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2012-09-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.571, 102.600, 138.032 |
| Unit cell angles | 90.00, 114.52, 90.00 |
Refinement procedure
| Resolution | 47.861 - 3.443 |
| R-factor | 0.2046 |
| Rwork | 0.202 |
| R-free | 0.24920 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.507 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.450 |
| Number of reflections | 41030 |
| <I/σ(I)> | 10.4 |
| Completeness [%] | 99.9 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2 M lithium chloride, 0.1 M Tris pH 8.0, 14% (w/v) PEG 3350 |
