5Y8V

Crystal structure of GAS41

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	55.467, 57.212, 205.854
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.495 - 2.610
R-factor	0.239
Rwork	0.237
R-free	0.27950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fk3
RMSD bond length	0.013
RMSD bond angle	1.501
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.600
Number of reflections	19176
<I/σ(I)>	9.51
Completeness [%]	98.7
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	291	0.1M MES pH 6.5, 1.6M MgSO4