5Y7O

Crystal structure of folding sensor region of UGGT from Thermomyces dupontii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2017-04-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97864
Spacegroup name	P 32 1 2
Unit cell lengths	195.090, 195.090, 142.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 3.100
R-factor	0.2352
Rwork	0.232
R-free	0.27830
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	2.269
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.290
High resolution limit [Å]	3.100	3.100
Rmerge	0.042	0.639
Number of reflections	105778	17378
<I/σ(I)>	14.4	1.4
Completeness [%]	96.4	98.3
Redundancy	2.7	2.8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.95 M Ammonium sulfate, 0.1 M Tris-HCl (pH 7.0), 8 mM n-decanoylsucrose