5Y79
Crystal structure of the triose-phosphate/phosphate translocator in complex with 3-phosphoglycerate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 107.050, 165.330, 41.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.998 - 2.200 |
R-factor | 0.1926 |
Rwork | 0.191 |
R-free | 0.22920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5i20 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.637 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 38123 | |
<I/σ(I)> | 13.3 | 1.3 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 39.2 | 27 |
CC(1/2) | 0.994 | 0.698 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6 | 293 | PEG200, Na-citrate, 3K-citrate, 2Na(3-PGA) |