5Y78

Crystal structure of the triose-phosphate/phosphate translocator in complex with inorganic phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-16
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	106.550, 164.970, 41.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.866 - 2.100
R-factor	0.1963
Rwork	0.195
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i20
RMSD bond length	0.002
RMSD bond angle	0.505
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	43639
<I/σ(I)>	11.3	1.1
Completeness [%]	99.8	100
Redundancy	90.3	87
CC(1/2)	0.995	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293	PEG200, MES, (NH4)2HPO4
1	LIPIDIC CUBIC PHASE	6	293	PEG200, MES, (NH4)2HPO4