5Y6N
Zika virus helicase in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.275, 52.934, 64.483 |
| Unit cell angles | 90.00, 104.93, 90.00 |
Refinement procedure
| Resolution | 40.341 - 1.571 |
| R-factor | 0.1825 |
| Rwork | 0.181 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gjc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.867 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2148: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.091 | 0.990 |
| Rpim | 0.476 | 0.476 |
| Number of reflections | 111281 | 2762 |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 99.8 | 98.2 |
| Redundancy | 6.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 291.15 | 0.1M imidazole (pH 7.0), 20% v/v Jeffamine ED-2001 |
