5Y6H
Crystal structure of YcgR-N domain of YcgR from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-25 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 91.960, 91.960, 32.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.982 - 1.774 |
| R-factor | 0.1736 |
| Rwork | 0.171 |
| R-free | 0.19320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y6f |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.770 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.110 | 1.870 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.054 | 0.319 |
| Rpim | 0.017 | 0.100 |
| Number of reflections | 9774 | 1396 |
| <I/σ(I)> | 26.6 | 7 |
| Completeness [%] | 98.5 | 97.6 |
| Redundancy | 11 | 10.7 |
| CC(1/2) | 0.999 | 0.962 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M MES pH 5.6, 0.01M Magnesium chloride hexahydrate, 1.8M Lithium sulfate |
