5Y6H

Crystal structure of YcgR-N domain of YcgR from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-25
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	91.960, 91.960, 32.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.982 - 1.774
R-factor	0.1736
Rwork	0.171
R-free	0.19320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y6f
RMSD bond length	0.004
RMSD bond angle	0.770
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.110	1.870
High resolution limit [Å]	1.770	1.770
Rmerge	0.054	0.319
Rpim	0.017	0.100
Number of reflections	9774	1396
<I/σ(I)>	26.6	7
Completeness [%]	98.5	97.6
Redundancy	11	10.7
CC(1/2)	0.999	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1M MES pH 5.6, 0.01M Magnesium chloride hexahydrate, 1.8M Lithium sulfate