5Y5S
Structure of photosynthetic LH1-RC super-complex at 1.9 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.280, 143.810, 210.280 |
| Unit cell angles | 90.00, 90.74, 90.00 |
Refinement procedure
| Resolution | 46.919 - 1.900 |
| R-factor | 0.1831 |
| Rwork | 0.181 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wmm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.270 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12rc0_2787) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.919 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.103 | |
| Rpim | 0.035 | 0.694 |
| Number of reflections | 338604 | 33819 |
| <I/σ(I)> | 9.47 | 1.14 |
| Completeness [%] | 100.0 | 99.98 |
| Redundancy | 9.2 | 8 |
| CC(1/2) | 0.997 | 0.557 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | PEG 1450, calcium chloride, octyl-phosphocholine |
