5Y56

Fc mutant (K392D/K409D/D399K)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	CCD
Collection date	2013-05-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 65 2 2
Unit cell lengths	65.130, 65.130, 477.521
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.445 - 2.653
R-factor	0.264
Rwork	0.260
R-free	0.29770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dn2
RMSD bond length	0.009
RMSD bond angle	1.176
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.570
High resolution limit [Å]	2.650
Number of reflections	18054
<I/σ(I)>	50.6
Completeness [%]	95.2
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	1.5M (NH4)2SO4, 10% Glycerol, 0.1M Tris-Hcl (pH 7.5)