5Y3D
Structural insight into the interaction between RNA polymerase and VPg for norovirus replication
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.172, 159.729, 121.704 |
| Unit cell angles | 90.00, 97.24, 90.00 |
Refinement procedure
| Resolution | 47.235 - 3.140 |
| R-factor | 0.2045 |
| Rwork | 0.203 |
| R-free | 0.24090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qid |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.545 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.140 |
| Number of reflections | 69820 |
| <I/σ(I)> | 5.2 |
| Completeness [%] | 96.3 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 297.15 | 0.1 M cacodylate (pH 6.5), 1.0 M sodium citrate |
