5Y2K

A human antibody AF4H1L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-08
Detector	MAR CCD 165 mm
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	107.266, 90.119, 111.404
Unit cell angles	90.00, 102.38, 90.00

Refinement procedure

Resolution	41.630 - 2.100
Rwork	0.220
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4py8
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9-1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.100	2.100
Rmerge	0.058	0.343
Number of reflections	118899	11093
<I/σ(I)>	25.1	4.5
Completeness [%]	98.0	92.3
Redundancy	6	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	291	0.1 M Bicine pH9.0, 2% v/v 1,4-Dioxane, 10% w/v PEG20000