5Y1U

Crystal structure of RBBP4 bound to AEBP2 RRK motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	76.761, 59.452, 102.438
Unit cell angles	90.00, 94.33, 90.00

Refinement procedure

Resolution	36.762 - 2.141
R-factor	0.1713
Rwork	0.169
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gfc
RMSD bond length	0.007
RMSD bond angle	0.908
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.230
High resolution limit [Å]	2.140	4.630	2.150
Rmerge	0.098	0.035	0.661
Rmeas	0.113	0.040	0.773
Rpim	0.057	0.019	0.395
Number of reflections	50650
<I/σ(I)>	7.4
Completeness [%]	99.9	99.8	100
Redundancy	3.9	4.1	3.7
CC(1/2)		0.998	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	283	0.2M Ammonium sulfate, 0.1M MES monohydrate pH 6.5 , 30%(w/v) Polyethylene glycol monomethyl ether 5,000