5Y18

Crystal structure of DAXX helical bundle domain in complex with ATRX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	69.485, 107.904, 30.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.976 - 2.202
R-factor	0.188
Rwork	0.186
R-free	0.21920
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.530
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHARP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.077	0.053	0.413
Rmeas	0.084	0.058	0.457
Rpim	0.033	0.024	0.192
Total number of observations	38355
Number of reflections	6179	677	594
<I/σ(I)>	6.6
Completeness [%]	99.6	98.8	99.8
Redundancy	6.2	5.8	5.4
CC(1/2)		0.995	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.1M sodium citrate, pH6.5, 0.2M ammonium acetate, 30% PEG4000