5XZH
Vitamin D receptor with a synthetic ligand ADRO2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2015-06-17 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.665, 41.763, 42.010 |
Unit cell angles | 90.00, 95.58, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.000 |
R-factor | 0.19683 |
Rwork | 0.194 |
R-free | 0.24338 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w5p |
RMSD bond length | 0.016 |
RMSD bond angle | 1.897 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 18042 | |
<I/σ(I)> | 7.6 | 1.3 |
Completeness [%] | 99.2 | 99.4 |
Redundancy | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MOPS/NaOH, 0.2M sodium formate, 14-18%(w/v) PEG4000, 8% (v/v) ethylene glycol |