5XZG

Mouse cGAS bound to the inhibitor RU521

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	PIXEL
Collection date	2016-12-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	85.562, 98.430, 130.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.215 - 1.828
R-factor	0.1958
Rwork	0.194
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.153
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.215
High resolution limit [Å]	1.828	1.828
Number of reflections	45239
<I/σ(I)>	17.7
Completeness [%]	92.3
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES, pH 6.9, 22.5% PEG400, 0.08 M magnesium chloride