5XZB
Mouse cGAS bound to the inhibitor RU365
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2015-08-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.008 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 85.502, 98.841, 130.201 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.726 - 2.130 |
| R-factor | 0.2149 |
| Rwork | 0.213 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.228 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.726 | |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 30303 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES, pH 6.3, 26% PEG400, 0.1 M magnesium chloride |
