5XZ3
The X-ray structure of Apis mellifera PGRP-SA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.898, 68.607, 71.699 |
| Unit cell angles | 90.00, 94.92, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.860 |
| R-factor | 0.19138 |
| Rwork | 0.190 |
| R-free | 0.21655 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s2j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.357 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.010 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 50577 |
| <I/σ(I)> | 9.4 |
| Completeness [%] | 91.3 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M TRIS hydrochloride; 2.0 M Ammonium sulfate; PH 8.5 |
