5XXP
Crystal structure of CbnR_DBD-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | AREA DETECTOR |
| Collection date | 2016-11-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.457, 28.719, 73.153 |
| Unit cell angles | 90.00, 111.65, 90.00 |
Refinement procedure
| Resolution | 67.990 - 2.550 |
| R-factor | 0.22164 |
| Rwork | 0.219 |
| R-free | 0.26327 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k1m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.366 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.990 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 11461 | 3253 |
| <I/σ(I)> | 13.52 | 3.27 |
| Completeness [%] | 97.1 | 95.5 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30%(w/v) PEG4000, 0.1 M Tris-HCl pH 8.5, 0.2 M sodium acetate |
