5XX8
Hetero-micro-seeding: Crystal structure of BPTI-[555]C14GA38I variant using micro-seeds from -C14GA38L variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 101 |
| Detector technology | CCD |
| Collection date | 2010-11-26 |
| Detector | AGILENT EOS CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.279, 41.479, 53.971 |
| Unit cell angles | 90.00, 99.35, 90.00 |
Refinement procedure
| Resolution | 53.250 - 1.300 |
| R-factor | 0.16471 |
| Rwork | 0.162 |
| R-free | 0.20647 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zvx |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.424 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.250 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.280 | |
| Number of reflections | 27383 | |
| <I/σ(I)> | 4.23 | 4.23 |
| Completeness [%] | 96.9 | 98.8 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20-30%PEG4000; 0.2M Lithium Sulfate; 0.1M Tris-HCl, pH8.5 |
