5XX6
Hetero-micro-seeding: Crystal structure of BPTI-[5,55]C14GA38I variant using micro-seeds from -C14GA38G variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 101 |
| Detector technology | CCD |
| Collection date | 2010-11-26 |
| Detector | AGILENT EOS CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.163, 41.284, 53.928 |
| Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
| Resolution | 53.220 - 1.310 |
| R-factor | 0.18063 |
| Rwork | 0.179 |
| R-free | 0.20951 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zvx |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.620 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.220 | 1.360 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmerge | 0.289 | |
| Number of reflections | 26484 | |
| <I/σ(I)> | 4.34 | 4.34 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20-30% PEG4000; 0.2M Lithium Sulfate; 0.1M Tris-HCl pH8.5 |
