5XX4
A BPTI-[5,55] variant with C14GA38K mutations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 101.15 |
| Detector technology | CCD |
| Collection date | 2010-11-26 |
| Detector | AGILENT EOS CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.201, 41.171, 54.742 |
| Unit cell angles | 90.00, 100.44, 90.00 |
Refinement procedure
| Resolution | 53.840 - 1.670 |
| R-factor | 0.14566 |
| Rwork | 0.142 |
| R-free | 0.22039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zvx |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.037 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.840 | 1.730 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.223 | |
| Number of reflections | 12943 | |
| <I/σ(I)> | 4.23 | 4.87 |
| Completeness [%] | 99.4 | 95.9 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 30% PEG4000; 0.2M Lithium Sulfate; 0.1M Tris-HCL, pH8.5 |
