5XX0

Crystal structure of the four N-terminal immunoglogulin domains of Sidekick-2 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2013-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	82.356, 86.801, 108.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.801 - 2.400
R-factor	0.2314
Rwork	0.228
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xwx
RMSD bond length	0.004
RMSD bond angle	0.845
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.065	0.440
Number of reflections	30490	2890
<I/σ(I)>	22.21	2.67
Completeness [%]	97.8	93.56
Redundancy	4.7	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M MgCl2, 0.1M Bis-Tris, pH5.5, 25% PEG 3350