5XWP
Crystal structure of LbuCas13a-crRNA-target RNA ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-21 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.576, 132.870, 139.960 |
| Unit cell angles | 90.00, 90.80, 90.00 |
Refinement procedure
| Resolution | 48.179 - 3.086 |
| R-factor | 0.2197 |
| Rwork | 0.218 |
| R-free | 0.24370 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.594 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.130 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rmerge | 0.185 | 0.558 |
| Number of reflections | 61255 | |
| <I/σ(I)> | 14 | 3.5 |
| Completeness [%] | 98.6 | 98.6 |
| Redundancy | 15 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M HEPES, pH 7.5, 0.1 M L-Proline, 8% PEG 3350 |
