5XWC
Crystal Structure of Aspergillus niger Glutamate Dehydrogenase Complexed With Alpha-iminoglutarate, 2-amino-2-hydroxyglutarate and NADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9537 |
Spacegroup name | H 3 2 |
Unit cell lengths | 173.410, 173.410, 241.830 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.000 - 1.750 |
R-factor | 0.15613 |
Rwork | 0.155 |
R-free | 0.16921 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.654 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.850 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.110 | |
Number of reflections | 138857 | |
<I/σ(I)> | 13.4 | 1.9 |
Completeness [%] | 99.0 | 99.9 |
Redundancy | 7.9 | 7.9 |
CC(1/2) | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 40% v/v PEG 300, 0.1M sodium cacodylate, pH 6.5, 0.2 M calcium acetate hydrate |