5XW0
Crystal Structure of Aspergillus niger Glutamate Dehydrogenase Complexed With Isophthalate and NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9763 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 173.480, 173.480, 241.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.16867 |
| Rwork | 0.168 |
| R-free | 0.18038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.444 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.054 | 0.420 |
| Number of reflections | 109208 | 15446 |
| <I/σ(I)> | 24.3 | 4.5 |
| Completeness [%] | 99.9 | 98.8 |
| Redundancy | 7.4 | 6.9 |
| CC(1/2) | 0.990 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 0.1 M MES (pH 6.0) 30% (v/v) PEG 200, 5 % (w/v) PEG 3000 |
