5XVP
E. fae Cas1-Cas2/prespacer/target ternary complex revealing the fully integrated states
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 64.870, 212.294, 512.199 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.030 - 3.000 |
| R-factor | 0.19557 |
| Rwork | 0.194 |
| R-free | 0.23264 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.580 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 68246 |
| <I/σ(I)> | 8.6 |
| Completeness [%] | 99.1 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.5 | 100 mM sodium acetate, 100 mM sodium citrate, pH 4.8, and 4-7% (w/v) PEG 4000 |
