5XVO

E. fae Cas1-Cas2/prespacer/target ternary complex revealing DNA sampling and half-integration states

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.91910
Spacegroup name	P 1 21 1
Unit cell lengths	131.893, 124.800, 157.905
Unit cell angles	90.00, 106.50, 90.00

Refinement procedure

Resolution	48.950 - 3.100
R-factor	0.19587
Rwork	0.194
R-free	0.23334
RMSD bond length	0.012
RMSD bond angle	1.576
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.100
Number of reflections	82547
<I/σ(I)>	13.2
Completeness [%]	97.3
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.5	100 mM sodium acetate, 100 mM sodium citrate, pH 6.2, and 4-7% (w/v) PEG 4000