5XVI

Crystal Structure of Aspergillus niger Apo- Glutamate Dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-08-15
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	92.800, 92.820, 111.690
Unit cell angles	103.46, 94.17, 120.13

Refinement procedure

Resolution	35.000 - 2.800
R-factor	0.22987
Rwork	0.226
R-free	0.31011
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sbo
RMSD bond length	0.010
RMSD bond angle	1.429
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.060	0.280
Number of reflections	69072	6502
<I/σ(I)>	11.2	3.6
Completeness [%]	92.0	86
Redundancy	2	1.6
CC(1/2)	0.990	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	20% PEG 3350, 0.1 M NaCl, 0.1 M Tris-Cl, pH 8.5, 0.01 M BaCl2