5XV7
SRPK1 in complex with Alectinib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2016-03-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 74.767, 74.767, 310.687 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.390 - 2.320 |
| R-factor | 0.21345 |
| Rwork | 0.212 |
| R-free | 0.23797 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wak |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.395 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 63.390 |
| High resolution limit [Å] | 2.320 |
| Number of reflections | 316057 |
| <I/σ(I)> | 33.67 |
| Completeness [%] | 99.1 |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 11% PEG 4000 100mM sodium citrate (pH 5.6) 200mM ammonium acetate |
