5XSF

Crystal structure of the 2-keto-3-deoxy-6-phosphogluconate aldolase of Zymomonas mobilis ZM4 with 3-phosphoglycerate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-14
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00
Spacegroup name	F 41 3 2
Unit cell lengths	186.299, 186.299, 186.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.853 - 1.962
R-factor	0.1916
Rwork	0.191
R-free	0.21210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xse
RMSD bond length	0.004
RMSD bond angle	0.658
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.853	1.990
High resolution limit [Å]	1.962	1.960
Number of reflections	20372
<I/σ(I)>	17.8	3.4
Completeness [%]	99.5	100
Redundancy	13.2	18.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.6M Ammonium Sulfate, 0.1M CAPS (pH 10.5), 0.2M Lithium Sulfate